Genomics Services Laboratory

Overview of Services

The Institute for Genomic Medicine Genomics Services Laboratory (GSL), located within the Research Institute at Nationwide Children's Hospital, is a core resource providing advanced sequencing technologies and comprehensive genomic services to support both internal and external researchers. Our expertise spans a range of high-throughput genomic assays, and we offer personalized support in experimental design, quality control for DNA/RNA starting materials, sample preparation, next-generation sequencing (NGS), and initial data processing.

Library preparation services include a variety of sequencing applications to meet diverse research needs. These include genome and exome sequencing, bulk RNA-seq, small RNA sequencing, microbial and metagenomic whole genome and RNA-seq, as well as multiple single-cell applications using 10x Genomics Chromium (3' Gene Expression, 5' Gene Expression + V(D)J enrichment, Fixed RNA Gene Expression, and ATAC + Gene Expression multiomics). Spatial transcriptomics is also available via 10x Genomics Visium technology. Additionally, we support epigenomic studies with ChIP-Seq and ATAC-Seq, facilitated by Hamilton Starlet automation for precise, high-throughput sample processing.

Sequencing services, the GSL is equipped with multiple Illumina platforms, including four NovaSeq6000 systems, which deliver high-throughput, flexible sequencing options with various flow cell types and read lengths (50–250 base pairs). We also offer lower-throughput sequencing using the Illumina NextSeq2000, MiSeq, and iSeq systems. Short-read sequencing is further supported by the Singular Genomics G4 instrument. For long-read applications, we offer library generation and sequencing using Pacific Biosciences Revio system.

The Steve and Cindy Rasmussen Institute for Genomic Medicine (IGM) is supported by the Nationwide Foundation Pediatric Innovation Fund. Publications resulting from data generated in IGM Genomic Services Core should cite the Nationwide Foundation Pediatric Innovation Fund.

Contact Information

Katherine Miller, PhD, Director

Amy Wetzel, PhD, Operations Manager

Phone: 614-722-5321 (select option 2 for GSL)

Office: 614-355-3530

Email: IGM Genomic Services <IGMGenomicServices@nationwidechildrens.org>

Location and hours of operation

Hours

Location

Mon - Fri

8:00 a.m. - 4:30 p.m.

The Abigail Wexner Research Institute at Nationwide Children's Hospital

575 Children's Crossroads, 2nd Floor (Research Building 3), WB2160

Columbus, Ohio 43215

How to Submit Samples

1. Initiate and sumit your request via iLab

Login or register for an iLab account: https://nch.ilab.agilent.com/account/login
Under Core Facilities, select Nationwide Children’s Hospital (NCH) and Genomics Services Laboratory (GSL).
Initiate “Next Generation Sequencing Request”.
Enter the project description information on NGS form.
The GSL will issue a quote upon completion of your request.
Agree to quote and provide payment information.
Payment information must be supplied before work on samples will begin.

2. Complete Project Intake Form (PIF) and Sample Submission Form (SSF)

IGM PIF and SSF (https://redcap.nchri.org/surveys/?s=WDR8XY9C7AM9HKKM)
Of note, the SSF should only be completed once the iLab order has been submitted

3. Non-NCH Users

Non-NCH users must also sign a master service agreement (MSA) or confirm with the GSL that an institutional MSA is currently in place and attach a hard copy of the purchase order (PO) to their iLab order prior to completing the SSF.

4. Project or Sample Related Communication

Email IGMGenomicServices@nationwidechildrens.org for questions regarding service offerings and delivery of samples.

5. Ship or Delivery of your Samples

If delivering your samples in person, please contact IGM Genomic Services to schedule a specific day and time.
If shipping samples, please ship to address below.

Institute for Genomic Medicine

Attn: IGM Research Sample Receipt and Accessioning Team

Nationwide Children’s Laboratory Services

700 Children’s Drive, Room C1955

Columbus, OH 43205 U.S.A.

If shipping your samples Additionally, please send the FedEx tracking number and include a shipping manifest with sample information (identifiers, specimen type, etc.) to the IGM Genomic Services group email listed above to ensure efficient receipt and triaging of samples.

Sample Submission Guidelines

Please submit samples in Eppendorf 1.5 ml LoBind tubes (Fisher Sci; Cat#13-698-791), or Eppendorf™ twin.tec™ 96 Well LoBind PCR Plates, Skirted (Fisher Sci; Cat# E0030129512). Tubes should be clearly labeled and readable with GSL order number, sample identifier, and lab name/PI.
For RNA/DNA QC services, submit samples in 0.5mL PCR strip tubes.
Samples may be suspended in dH2O, 10mM Tris, or Qiagen EB. High salt (EDTA) concentration can interfere with the various enzymes that are used during library preparation. Our preference is that samples are suspended in dH2O.

Library Preparation Services for Illumina Short-Read Sequencing:

Service	Illumina Library Prep Method	Input Material	Concentration (ng/µL) using Qubit	Minimum Volume Required (µL)	Recommended Amount (ng)
Human Whole Exome Sequencing (WES)	NEBNext UltraII FS, IDT xGEN Exome Research Panel v2 + CNV Probes	Genomic DNA	>25	20	≥200
Mouse Whole Exome Sequencing (WES)	TWIST EF Library + Capture	Genomic DNA	>20	20	≥200
Whole Genome Sequencing (WGS)	NEBNext Ultra II FS	Genomic DNA	>25	20	≥500
FFPE DNA -WGS/WES	SRSLY NanoPlus Library Prep	Genomic DNA	>10	20	≥100
Total Stranded RNA-Seq	NEBNext UltraII Directional	Total RNA	>50	20	≥500
miRNA-Seq	QIASeq miRNA	Total RNA including small RNA fractions (>17 nt)	>50	25	≥1000

Library Preparation Services for PacBio Long-Read Sequencing

Service	Input Material	Concentration (ng/µL) using Qubit	Minimum Volume Required (µL)	Recommended Amount (ng)
Genome (HiFi)	Genomic DNA with 90% >30Kb	>100	50	≥3000
Plasmid	Plasmid DNA Linearized	>50	50	≥1000
IsoSeq RNA	RNA (DNAsed) with RIN >7	>50	20	≥600
Kinnex RNA	RNA (DNAsed) with RIN >7			≥600
Kinnex scRNA	High quality cDNA from 10X	>2	15	≥30

Sample Submission Guidelines for Sequencing Services (Client-Submitted Libraries that do NOT require DNA Library QC)

Sequencer	Sample Type	Minimum Volume (µL)	Concentration
PacBio Revio SMRT Cell	Final Library	50	>20 ng/µL
Illumina NextSeq 2000	Final Library	80	2 nM
Illumina MiSeq	Final Library	20	10 nM
Illumina NovaSeq 6000: SP Flow Cell	Final Library	220	1.5 nM
Illumina NovaSeq 6000: SP-Xp (2-lane segregation)	Final Library	45 per lane	1 nM
Illumina NovaSeq 6000: S1- Flow Cell	Final Library	220	1.5 nM
Illumina NovaSeq 6000: S1-Xp (2-lane segregation)	Final Library	45 per lane	1 nM
Illumina NovaSeq 6000: S2- Flow Cell	Final Library	320	1.5 nM
Illumina NovaSeq 6000: S2-Xp (2-lane segregation)	Final Library	60 per lane	1 nM
Illumina NovaSeq 6000: S4- Flow Cell	Final Library	640	1.5 nM
Illumina NovaSeq 6000: S4-Xp (4-lane segregation)	Final Library	70 per lane	1 nM

Website

https://www.nationwidechildrens.org/specialties/institute-for-genomic-medicine/genomic-services